Summary
FPC (fingerprinted contigs) is an interactive program for building
contigs from fingerprinted clones, where the fingerprint for a clone
is a set of restriction fragments. FPC has an algorithm to automatically
cluster clones into contigs based on their probability of coincidence
score. For each contig, it builds a consensus band (CB) map which
is similar to a restriction map but it does not try to resolve all
the errors. The CB map is used to assign coordinates to the clones
based on their alignment to the map and to provide a detailed visualization
of the clone overlap. FPC has editing facilities for the user to refine
the coordinates and to remove poorly fingerprinted clones. Functions
are available for updating an FPC database with new clones. Contigs
can easily be merged, split or deleted. Markers can be added to clones
and are displayed with the appropriate contig. Sequence ready clones
can be selected and their sequencing status displayed. As such, FPC
is an integrated program for the assembly of sequence ready clones
for large scale sequencing projects.
For agarose
gels, the program Image
is typically used to produce our bands files.
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